URL: http://www.addgene.org/21715
Proper Citation: RRID:Addgene_21715
Insert Name: ROSA26-5' insert
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9916792
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3027; Vector Backbone:pBluescript KS (-); Vector Types:for use with mouse targeting vector pROSA26-1; Bacterial Resistance:Ampicillin
Comments: Probe for detecting targeted integrations by Southern blots. It is a small probe so users should wash at low stringency, eg. 1xSSC at 65 degrees. We recommend you order this plasmid along with pROSA26-1 and SAbgeo as a "ROSA26 targeting kit". For more information visit - http://research.mssm.edu/soriano/lab
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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