URL: http://www.addgene.org/24225
Proper Citation: RRID:Addgene_24225
Insert Name: E2F1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8622649
Vector Backbone Description: Backbone Size:6200; Vector Backbone:pCMVHANeoBam; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Insert check with SalI, which leads to a 1.3kb fragment + vector. E2F1 cDNA can be removed by BamHI digest. This plasmid contains an E356K mutation within the E2F1 insert. Please note that other mutations may exist and it is unclear whether this mutation affects plasmid function.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
