URL: http://www.addgene.org/20183
Proper Citation: RRID:Addgene_20183
Insert Name: Notch 1 Intracellular Domain
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16365048
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:p3XFLAG-CMV-7; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: All NICD plasmids were cloned into the p3XFLAG-CMV-7(TM) expression vector (Sigma). NICD1 was cloned into XbaI/BamHI sites. NICD1 (Val-1744 to Lys-2531 of full-length mouse N1) was amplified using primers 5XbaN1 (5'-GCCTCTAGAGTGCTGCTGTCCCGCAAGCGC-3') and 3BamN1 (5'-CGGGATCCTTATTTAAATGCCTCTGGAATGTGGG-3').
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Source: Addgene