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URL: https://web.expasy.org/cellosaurus/CVCL_HC52
Proper Citation: Kerafast Cat# ENH104-FP, RRID:CVCL_HC52
Description: Cell line NIH 3T3 Duo-BFP53BP1 is a Spontaneously immortalized cell line with a species of origin Mus musculus (Mouse)
Sex: Male
Defining Citation: PMID:23929981
Comments: Characteristics: Integrates one copy of a LacO-ISceI construct (256 copies of LacO), two copies of a TetO-ISceI-TetO construct (96 copies of TetO) as well as a LacR-GFP and a TetR-mCherry constructs., Characteristics: Cell developed for the induction and visualization of double-strand breaks chromosomal damage at specific sites of the mammalian genome. Sytem based on the stable integration of endonuclease ISceI recognition sites flanked by LacO/TetO operator arrays, coupled with retroviral-mediated integration of their fluorescent repressors (LacR/TetR) to visualize the LacO/TetO sites.
Category: Spontaneously immortalized cell line
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Source: Cellosaurus
