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URL: https://web.expasy.org/cellosaurus/CVCL_ZC43
Proper Citation: RRID:CVCL_ZC43
Description: Cell line 4B6/3598/3442#2/4011C1 is a Transformed cell line with a species of origin Mus musculus (Mouse)
Sex: Sex unspecified
Defining Citation: PMID:23884459
Comments: Characteristics: Transfected with reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16., Characteristics: Unlike the parental cell line, this cell line maintains mtDNA at normal levels. This was achieved by introducing into the parental cell line a ligA gene fused to an excisable (FRT/FLPo) mitochondrial targeting sequence (MTS) from human ornithine transcarbamylase. The resulting cell line expresses two ligA variants: one without- and another with MTS. The latter is efficiently imported into mitochondria and therefore normal mtDNA copy number is restored in the transduced cells (Kerafast=ESA104).
Category: Transformed cell line
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Source: Cellosaurus
