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URL: http://antibodyregistry.org/AB_10726367
Proper Citation: GeneTex Cat# GTX89083, RRID:AB_10726367
Target Antigen: PIAS2 antibody
Host Organism: goat
Clonality: polyclonal
Comments: Discontinued; manufacturer recommendations: Immunohistochemistry; ELISA; Preliminary experiments gave an approx 90kDa band in lysates of cell line A431after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 68.2kDa according to NP_004662.2. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? In paraffin embedded Human Testis shows nuclear and extra-nuclear staining in spermatids. Recommended concentration, 3-5µg/ml. This antibody is expected to recognise both reported isoforms (NP_775298.1 and NP_004662.
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No rating or validation information has been found for PIAS2 antibody.
Discontinued
Discontinued at GeneTex
Source: Antibody Registry