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C.B. Pert, National Institute of Mental Health, Bethesda, Maryland Cat# Bombesin-GRP, RRID:AB_2783525Copy Citation Copied
URL: http://antibodyregistry.org/AB_2783525
Proper Citation: C.B. Pert, National Institute of Mental Health, Bethesda, Maryland Cat# Bombesin-GRP, RRID:AB_2783525
Target Antigen: bombesin
Host Organism: rabbit
Clonality: unknown
Comments: "Antibody Elicitation. Immunogens were prepared using the method of Goodfriend et al. (14). -- Five mg of synthetic [Lys3]BN (Peninsula Laboratories, San Carlos, CA) was coupled to 10 mg of bovine serum albumin using 100 mg of ethyl-3-(3-dimethyl-amino propyl) carbodimide hydrochloride. After a 15 min. incubation unconjugated peptide and excess carbodimide were removed by dialysis against water. The conjugated peptide was emulsified in an equal volume of Freund's complete adjuvant (Calbiochem-Behring Corp., La Jolla, CA) and the rabbits were injected in the dermis of the back at multiple sites. The rabbits were boosted at monthly intervals with conjugated peptide emulsified with Freund's incomplete adjuvant. Three months after the initial injection the rabbits were boosted with 100 mg of aluminum oxide (ICN Pharmaceuticals, Inc., Cleveland, OH) which was precoated with 0.5 mg of BN. Sera which contained antibodies were frozen at -8O'C and thawed just prior to use."
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Source: Antibody Registry