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URL: http://antibodyregistry.org/AB_371949
Proper Citation: GeneTex Cat# GTX10766, RRID:AB_371949
Target Antigen: IL7 antibody
Host Organism: goat
Clonality: polyclonal
Comments: Discontinued; manufacturer recommendations: IgG; IgG ELISA: Use at a concentration of 0.5 - 1.0 µg/ml. Neut: Use at an assay dependent dilution. WB: Use at a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 15 kDa. The Neutralization Dose 50 (ND50) for anti-mouse IL7 is approximately 0.2-0.4µg/ml in the presence of 1.5 ng/ml of recombinant mouse IL7 using human peripheral blood mononuclear cells activated with PHA for 5 days. The exact concentration of antibody required to neutralize the recombinant mouse IL7 activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity. The detection limit in immunoblotting for recombinant mouse IL7 is approximately 5.0 ng/lane under non-reducing and reducing conditions. The detection limit in ELISA for recombinant mouse IL7 is
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No rating or validation information has been found for IL7 antibody.
Discontinued
Discontinued at GeneTex
Source: Antibody Registry
