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URL: http://antibodyregistry.org/AB_374030
Proper Citation: GeneTex Cat# GTX10755, RRID:AB_374030
Target Antigen: IL4 antibody
Host Organism: goat
Clonality: polyclonal
Comments: Discontinued; manufacturer recommendations: IgG; IgG ELISA; Block/Neutralize/Inhibit; Western Blot; ELISA, Neutralizing, Western blot. ELISA: Use at a concentration of 0.5 - 1 µg/ml, this detects 0.3 ng/well of rhIL4. Neut: Use at a concentration of 0.004 - 0.012 µg/ml. WB: Use at a concentration of 0.1 - 0.2 µg/ml, this detects rhIL2 at 2 ng/lane under non-reducing and reducing conditions. Predicted molecular weight: 20 kDa. Anti-Human IL4 is tested for its ability to neutralize the bioactivity of rhIL4 in a cell proliferation assay using a human factor-dependent cell line, TF1. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhIL4 that is present at a concentration just high enough to elicit a maximum response. In this bioassay, rhIL4 was
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No rating or validation information has been found for IL4 antibody.
Discontinued
Discontinued at GeneTex
Source: Antibody Registry
