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URL: http://antibodyregistry.org/AB_381146
Proper Citation: GeneTex Cat# GTX10749, RRID:AB_381146
Target Antigen: IL1 beta antibody [8516.311]
Host Organism: mouse
Clonality: monoclonal
Comments: Discontinued; manufacturer recommendations: IgG1; IgG1 Block/Neutralize/Inhibit; Immunohistochemistry; Western Blot; ELISA; ELISA: Use at an assay dependent dilution. Neut: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 31 kDa. Monoclonal Anti-Human IL1 beta is tested for its ability to neutralize the biological activity of rhIL1 beta on the mouse T-helper D10. G4.1 cell line. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhIL1 beta that is present at a concentration just high enough to elicit a maximum response. In this bioassay, 50 pg/ml rhIL1 beta was mixed with various dilutions of antibody in a 96-well plate for 1 hour at 37 °C. After preincubation, D10. G4 cells and 1.25 µg/ml concanavalin
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No rating or validation information has been found for IL1 beta antibody [8516.311].
Discontinued
Discontinued at GeneTex
Source: Antibody Registry
