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URL: http://antibodyregistry.org/AB_10821224
Proper Citation: abcepta Cat# AP10250b, RRID:AB_10821224
Target Antigen: SLC10A4
Host Organism: rabbit
Clonality: polyclonal
Comments: Applications: ELISA, Western Blot
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No rating or validation information has been found for SLC10A4 Antibody (C-term).
Possibly Non-Specific
Extracted Antibody Information: "or Abgent rabbit anti-SLC10A4 (1:500 dilution) [Abgent Cat# AP10250b RRID:AB_10821224],"
Extracted Specificity Statement: "They used the human medulloblastoma cell line TE671 in which they localized the SLC10A4 protein by immunofluorescence and Western Blot analysis with a commercial anti-SLC10A4 antibody and showed that pre-incubation of the cells with 1U/200 µl of thrombin for 3 h increased the cellular uptake of taurocholic acid and lithocholic acid [18]. However, appropriate controls demonstrating specificity of the antibody were not provided and the authors failed to seriously show saturable transport kinetics for this uptake. Furthermore, bioinformatics analysis (Expasy Peptide Cutter, http://www.web.expasy.org/peptide_cutter/) indicates that the mouse and rat SLC10A4 proteins can be cleaved by thrombin at amino acid position 87, but not the human SLC10A4 protein."
Data was mined by Antibody Watch (https://arxiv.org/pdf/2008.01937.pdf), from PMID:26084360
Source: Antibody Registry
