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URL: http://antibodyregistry.org/AB_2722597
Proper Citation: Fukagawa Lab - University of Osaka Cat# Fukagawa_lab_009, RRID:AB_2722597
Target Antigen: Hec1
Host Organism: rabbit
Clonality: polyclonal
Comments: "A chicken Nuf2 expression construct (amino acids 91-293) and a chicken Hec1 expression construct (amino acids 465-640) were created in vector pET28a (Novagen). The histidine-tagged recombinant protein was expressed in E. coli BL21 (DE3) cells. Purified recombinant proteins were used to immunize a rabbit. Serums were affinity purified against recombinant proteins on a CNBr-activated Sepharose 4B column. Immunoprecipitation was performed by a standard method."
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Source: Antibody Registry
