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URL: http://antibodyregistry.org/AB_374557
Proper Citation: GeneTex Cat# GTX28944, RRID:AB_374557
Target Antigen: Salivary Amylase antibody
Host Organism: sheep
Clonality: polyclonal
Comments: Discontinued; manufacturer recommendations: IgG; IgG Immunohistochemistry; Other; Western Blot; ELISA; Immunodiffusion; For western blotting: Antigen: 50 salivaAntibody: 1:5000Secondary Antibody: 1:1000 Donkey a-Sheep/Goat immunoglobulins-peroxidaseDouble diffusion: 10µL antiserum vs 5µL salivaIEP: 100µL antiserum vs 5µL saliva Results can be enhanced by washing completed agarose gels in saline, drying and finally stainingwith a protein stain such as coomassie blue. The use of 3 PEG 6000 with 1.2 agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Optimal dilutions should be determined by the individual laboratory Suitability for use in nephelometry, immunohistochemical procedures and enzyme-linked immunosorbent assays has notbeen assessed but use in such assays should not necessarily
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No rating or validation information has been found for Salivary Amylase antibody.
Discontinued
Discontinued at GeneTex
Source: Antibody Registry
