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URL: http://antibodyregistry.org/AB_381088
Proper Citation: GeneTex Cat# GTX10580, RRID:AB_381088
Target Antigen: Jagged 1 antibody
Host Organism: goat
Clonality: polyclonal
Comments: Discontinued; manufacturer recommendations: IgG; IgG Immunofluorescence; Western Blot; Flow Cytometry; Block/Neutralize/Inhibit; ELISA; ELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant rat Jagged-1 is approximately 0.16 ng/well. FACS: Use at a concentration of 20 µg/ml. (10 µl/10^5 - 10^6 cells) this concentration is recommended to label human Jagged 1 transfected FBM stromal cells. Neut: Use at a concentration of 2 - 10 µg/ml. The antibody will block 50 of the binding of 200 ng/ml of recombinant rat Jagged-1/Fc to immobilized recombinant rat Notch-1/Fc or recombinant rat Notch-2/Fc (100 µl of a 5 µg/ml solution coated in each well) in an ELISA assay. WB: Use at a concentration of 0.1 - 0.2 µg/ml, this concentration is determined using rat jagged-1 at 5 ng/lane und
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No rating or validation information has been found for Jagged 1 antibody.
Discontinued
Discontinued at GeneTex
Source: Antibody Registry