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URL: http://antibodyregistry.org/AB_422928
Proper Citation: GeneTex Cat# GTX17869, RRID:AB_422928
Target Antigen: p53 [3F301] antibody
Host Organism: mouse
Clonality: monoclonal
Comments: Discontinued; manufacturer recommendations: Immunohistochemistry; Immunohistochemistry - fixed; Immunofluorescence; Flow Cytometry; Western Blot; Immunoprecipitation; FACS: Use at an assay dependent dilution. IHC-P: Use at a concentration of 2 - 4 µg/ml.Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. IP: Use at a concentration of 2 µg/mg lysate (GTX17869 will detect both native and denatured forms of the target, use protein G beads). WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 53 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user., FACS, IHC-P, IP, WB, Flow cytometry/FACS, IHC (Formalin-fixed paraffin-embedded sections),
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No rating or validation information has been found for p53 [3F301] antibody.
Discontinued
Discontinued at GeneTex
Source: Antibody Registry
