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URL: http://antibodyregistry.org/AB_625832
Proper Citation: GeneTex Cat# GTX80798, RRID:AB_625832
Target Antigen: VR1 antibody
Host Organism: guinea pig
Clonality: polyclonal
Comments: Discontinued; manufacturer recommendations: Immunohistochemistry; For IHC: Use at a dilution of 1:1,000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. Immunohistochemistry: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) 4 paraformaldehyde fixative, and 3) 10 sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10 sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4oC with primary antiser
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No rating or validation information has been found for VR1 antibody.
Discontinued
Discontinued at GeneTex
Source: Antibody Registry
