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URL: http://www.addgene.org/100098
Proper Citation: RRID:Addgene_100098
Insert Name: DNA fragmentation factor subunit alpha
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:26106156
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please note- insertion of amino acids ENLYFG after AA117, and insertion of ENLYFQSG after AA224 in ICAD [Genbank sequence NP_004392.1.] are expected insertions. They make the ICAD variant recognizable and cleavable by TEVP.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org