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URL: http://www.addgene.org/101342
Proper Citation: RRID:Addgene_101342
Insert Name: NL4-3
Bacterial Resistance: Ampicillin
Defining Citation: PMID:30546110
Vector Backbone Description: Vector Backbone:pNL4-4; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: The last methionine in Gag (M423) was left as it was outside the ApaI site used to clone in the mutations. However, C-terminus of Gag is functionally inert, and Pol slips 27 nucleotides away and is unlikely to produce a stable protein product.
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No rating or validation information has been found for pNL4-3 envFS eGFP NoStarts.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
