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URL: http://www.addgene.org/102270
Proper Citation: RRID:Addgene_102270
Bacterial Resistance: Tetracycline
Defining Citation: PMID:29164072
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Tetracycline
Comments: Genotype = ΔompA ΔompC ΔompF ΔlamB Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte] Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene. Due to the strain's growth defect and potential for contamination, Addgene recommends streaking for single colonies from the stab on low salt LB containing tetracycline and then screening colonies by PCR using the Leo Lab's recommended primers to confirm the strain.
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Source: Addgene
