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URL: http://www.addgene.org/105997
Proper Citation: RRID:Addgene_105997
Bacterial Resistance: Ampicillin
Defining Citation: PMID:29334369
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, Lentiviral, CRISPR, S.pyogenes sgRNA expression vector; Bacterial Resistance:Ampicillin
Comments: Dual sgRNA vector to express one sgRNA for S.pyogenes Cas9 and one sgRNA for S.aureus Cas9. Cloning is done in two steps: 1.) AarI digest to clone the S.pyogenes sgRNA and after preparation of the successfully cloned plasmid 2.) BfuAI digest followed by cloning of the S.aureus sgRNA.
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Source: Addgene
