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URL: http://www.addgene.org/109286
Proper Citation: RRID:Addgene_109286
Insert Name: GroEL-GFP
Organism: Other
Bacterial Resistance: Kanamycin
Defining Citation: PMID:22820329
Vector Backbone Description: Backbone Marker:from PMID 9218713; Vector Backbone:pSUM36; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: The groEL promoter was PCR-amplified from M. tuberculosis genomic DNA, cloned into the pCR2.1 TOPO-TA cloning vector and subsequently isolated as a BamHI/XbaI piece that was subcloned into pSUM36 cut with BamHI and NheI to remove the lacZ promoter. The GFP gene was subcloned into the HindIII site of the pSUM-groEL vector.
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Source: Addgene
