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URL: http://www.addgene.org/110406
Proper Citation: RRID:Addgene_110406
Insert Name: GLS glutaminase
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:#50005; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Homology arm for C-Terminal knock-in at stop codon of GLS' KGA isoform. Just mKO2 knock-in. Construction order: GLS 1kb 5' Homology Arm: SacI-BamHI; GLS 1kb 3' Homology Arm BamHI-SalI; BglII-BamHI mKO2 System options: sgRNA: pX330.puro sgRNA KGA Stop1 (Addgene #110405) Homology arms: pUC19 KGA HA5'-mKO2-3' (Addgene #110406) pUC19 KGA HA5'-mKO2-P2A-3' (Addgene #110407) pUC19 KGA HA5'-mKO2-P2A-Zeo-3' (Addgene #110408)
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Source: Addgene