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URL: http://www.addgene.org/11346
Proper Citation: RRID:Addgene_11346
Insert Name: gateway cassette
Bacterial Resistance: Chloramphenicol and Ampicillin
Vector Backbone Description: Backbone Marker:see map of pACT2.2 in "author's map"; Backbone Size:7550; Vector Backbone:pACT2.2; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway-modified yeast two-hybrid Activation domain vector. Use this vector for Gateway cloning of a cDNA to be used to test a directed yeast two-hybrid interaction. This vector was modified from plasmid pACT2.2 by placing a Gateway acceptor cassette in the SmaI site of pACT2.2. To facilitate subcloning of a given DNA insert into this plasmid, Gateway recombinational attachment sites should be incorporated into primers used for amplificiation, as outlined in the Invitrogen Gateway manual. (Gateway is a registered trademark of Invitrogen Corporation.) Please note that the "author's map" is the map of the original pACT2.2 vector upon which pACT2.2gtwy is derived - it is not a map of pACT2.2gtwy. This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
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Source: Addgene
