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URL: http://www.addgene.org/11586
Proper Citation: RRID:Addgene_11586
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15240889
Vector Backbone Description: Backbone Size:7703; Vector Backbone:pSico PGK puro; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pSico PGK puro allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression. The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website http://web.mit.edu/ccr/labs/jacks/protocols/pSico.html
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Source: Addgene
