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URL: http://www.addgene.org/117803
Proper Citation: RRID:Addgene_117803
Insert Name: CENP-A
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:11129795
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The insert fragment with Sac I and Xho I sites was cloned into Sac I and Sal I sites of pEGFP-C1. Thus, there is no Xho I and Sal I site in pEGFP-AF8. The stop codon was also changed to TCG (..ctcggcTCGac-3’) Note that the cloning strategy adds additional amino acids onto the C-terminus of the insert (STVPRARDPPDLDN), but this does not interfere with the function.
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Source: Addgene
