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URL: http://www.addgene.org/118154
Proper Citation: RRID:Addgene_118154
Insert Name: KRAB-dCas9-P2A-BlastR
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene #52961); Vector Backbone:lentiCRISPRv2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: We have also generated a version of this backbone with the hPGK promoter driving the expression of KRAB-dCas9. We advise users to test both backbones in their cell type of interest. Note: Plasmid contains a N1396S mutation in dCas9. This mutation is not known to affect plasmid function.
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Source: Addgene
