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URL: http://www.addgene.org/118224
Proper Citation: RRID:Addgene_118224
Insert Name: CENP-A
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:18778791
Vector Backbone Description: Backbone Marker:MBL, Nagoya, Aichi, Japan; Backbone Size:4656; Vector Backbone:pmKO1-MC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Bgl II-KpnI fragment of pEGFP-AF8 (Addgene plasmid # 117803) was recloned into BamHI and KpnI site of pmKO1-MC1. Note that the cloning strategy adds additional amino acids onto the C-terminus of the insert (STVPELQQRIRETREQSLNKRPRL), but this does not interfere with plasmid function. The extra sequence includes an MCS for adding tags to the C-terminus of the insert.
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Source: Addgene
