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URL: http://www.addgene.org/11911
Proper Citation: RRID:Addgene_11911
Insert Name: pRSETA-PAGFP
Organism: Aequorea victoria
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12228718
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2827; Vector Backbone:pRSETA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The fluorescent protein encoding region contains one of the three substitutions (A206K) suggested by Dr. Roger Tsien. This mutation disrupts fluorescent protein dimerization even at high concentrations. The mutation does not seem to have much effect on the fluorescent properties of the CFP, GFP and YFP versions, so hopefully it will not affect PAGFP too much. The PAGFP A206K mutant does not display obvious fluorescent differences compared with the original, but please bear in mind that I haven't characterized it to the same extent. The citation for the A206K mutation in fluorescent proteins is Science 2002 296:913.
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Source: Addgene
