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URL: http://www.addgene.org/120264
Proper Citation: RRID:Addgene_120264
Insert Name: pDYSCKO cassette-ADE1 targeting guide
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:30097473
Vector Backbone Description: Backbone Size:6467; Vector Backbone:pDYSCKO; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: See Double Selection Enhances the Efficiency of Target-AID and Cas9-Based Genome Editing in Yeast. (PMID: 30097473) for detailed protocols for gRNA cloning and mutagenesis protocol.
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Source: Addgene