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URL: http://www.addgene.org/120371
Proper Citation: RRID:Addgene_120371
Insert Name: Akaluc (human optimized)
Bacterial Resistance: Kanamycin
Defining Citation: PMID:34650390
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3928; Vector Backbone:N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The akaluc gene was synthesized with human/murine codon optimization from the protein amino acid sequenced provided by Iwano et. al. Science 2018 as a more sensitive luciferase enzyme for live animal imaging in vivo with the substrate Akalumine-HCl. After synthesis, the gene was cloned and cloned into the eGFP-N1 (Addgene 6085-1) using restriction enzyme cloning.
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Source: Addgene
