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URL: http://www.addgene.org/121842
Proper Citation: RRID:Addgene_121842
Insert Name: 3x HA epitope tag-PolyA + hU6-xCas9(3.7) gRNA scaffold
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:30590691
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2555; Vector Backbone:pDONR221 P3-P2; Vector Types:Gateway Cloning, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: Annealed oligonucleotides containing the protospacer motif can be ligated into BsaI digested plasmid. 3x HA tag + bGH polyA; hU6 promoter PCR'd from Addgene 61591. x/SpCas9 gRNA scaffold derived from PMID: 26671237
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No rating or validation information has been found for KK701: pMAGIC (L3-L2) 3x HA eptitope tag + polyA; hU6::xCas9(3.7) gRNA scaffold.
No alerts have been found for KK701: pMAGIC (L3-L2) 3x HA eptitope tag + polyA; hU6::xCas9(3.7) gRNA scaffold.
Source: Addgene
