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URL: http://www.addgene.org/122243
Proper Citation: RRID:Addgene_122243
Insert Name: C-terminal end of Nesprin-3 w/o Kash
Organism: Mus musculus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:34857921
Vector Backbone Description: Backbone Size:7469; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Comments: Dominant negative Kash construct is comprised of the C-terminal end of Nesprin-3, including the transmembrane but excluding the Kash domain, and fused to Nesprin2.5 for visual location and to the signal peptide of TOR1A (NM_000113.2) for membrane integration. This vector is meant as integration defective control (into LINC complex) for the vector Ad:2CMV_A2R2-N25K3 (122242). Please note the L451V substitution in Actinin alpha 2 has no functional consequences. Please visit https://www.biorxiv.org/content/early/2018/10/29/455600 for BioRxiv preprint.
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Source: Addgene
