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URL: http://www.addgene.org/122443
Proper Citation: RRID:Addgene_122443
Insert Name: reelin
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8987733
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The entire reelin open reading frame was assembled by fusing five overlapping cDNA clones isolated previously (D’Arcangelo et al., 1995) and subcloning them into pcDNA3. The following reelin fragments were used: 1.2 kb NaeI–SalI from p59BS1, 1.3 kb SalI–NdeI from pBS2, 3.1 kb NdeI–BspEI from pBS6, 770 bp BspEI–ApaLI from p3Rea3, and 4.2 kb ApaLIEcoRV from pBS53. The final clone (pCrl) contains the entire reelin open reading frame (10,383 bp) plus 95 bp of sequence 59 to the initiator methionine codon and 82 bp of 39 untranslated sequence (reelin cDNA nucleotides 188–10,748).
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Source: Addgene
