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URL: http://www.addgene.org/12392
Proper Citation: RRID:Addgene_12392
Insert Name: transferrin receptor
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Pharmingen; Backbone Size:9761; Vector Backbone:pAcGP67A; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: The murine TfR sequence bp 367-2292 (of the ORF) was cloned into the pAcGP67A vector. The construct includes a short 3' UTR (past the stop codon at bp 2292). The TfR fragment was PCR amplified to introduce an EcoRI site at the 5' end and an EagI site at the 3' end. This was subsequently cloned into the corresponding EcoRI/EagI sites of pAcGP67A. The murine TfR was fused 3' to a gene segment encoding the hydrophobic leader peptide from the baculovirus protein gp67, a 6X-His tag, and a factor Xa cleavage site.
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Source: Addgene
