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URL: http://www.addgene.org/124286
Proper Citation: RRID:Addgene_124286
Insert Name: Collagen type VII alpha 1 chain
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Backbone Size:9200; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: Plus "G" was routinely added because U6 promoter prefers "G" as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.
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No rating or validation information has been found for Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0).
No alerts have been found for Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0).
Source: Addgene
