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Plasmid Name
RRID:Addgene_124289 RRID Copied  
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RRID:Addgene_124289
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Plasmid Information

URL: http://www.addgene.org/124289

Proper Citation: RRID:Addgene_124289

Insert Name: NFATc4 nuclear factor of activated T cells

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 62988); Backbone Size:9200; Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0; Vector Types:CRISPR; Bacterial Resistance:Ampicillin

Comments: Plus "G" was routinely added because U6 promoter prefers "G" as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.

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Usage and Citation Metrics

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Ratings and Alerts

No rating or validation information has been found for NFATc4-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0).

No alerts have been found for NFATc4-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0).

Data and Source Information

Source: Addgene