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URL: http://www.addgene.org/12494
Proper Citation: RRID:Addgene_12494
Insert Name: CRE-M AR
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12888538
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCMV-Cre-M (BglII); Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: To generate a fusion gene consisting of cre recombinase and the AR LBD, plasmid p258 was digested with BglII and MluI and the hAR fragment coding for LBD was isolated. This fragment was cloned into pCMV-CRE-M(BglII) by digesting two separate aliquots of pCMV-CRE-M(BglII) with ScaI/BglII or with ScaI/MluI. The ligation of these three fragments resulted in the generation of pCMV-CRE-M-AR(wt).
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Source: Addgene