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Plasmid Name
pCAG-sfGFP
RRID:Addgene_125747 RRID Copied  
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RRID:Addgene_125747
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Plasmid Information

URL: http://www.addgene.org/125747

Proper Citation: RRID:Addgene_125747

Insert Name: sfGFP

Organism: Synthetic

Bacterial Resistance: Ampicillin

Defining Citation: PMID:32183906

Vector Backbone Description: Vector Backbone:Custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional. Nevertheless, for sfGFP expression, the depositor recommends the updated versions of this plasmid, PB CAG-sfGFP (https://www.addgene.org/133568/) or PB-CA sfGFP (https://www.addgene.org/159094/), that can also be used without the piggyBac transposase. Red fluorescent protein versions are also available: PB CAG-tdTomato (https://www.addgene.org/133569/) or PB-CA tdTomato (https://www.addgene.org/159095/).

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Data and Source Information

Source: Addgene