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URL: http://www.addgene.org/12615
Proper Citation: RRID:Addgene_12615
Insert Name: GB1 domain of protein G as N terminal tag, with Bcl2 as insert
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9385638
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: In GEV1, a noncleavable linker (Pro-Gly-Gly-Pro-Ala-Ser) is encoded between the GBl domain and the N-terminus of the protein of interest. Genes can be inserted into this vector using Nhe I and Xho I cloning sites, and, if no stop codons are introduced at the 5' end of an insert, the vector encodes a C-terminal poly His-tag. Also see: "A rapid method to attain isotope labeled small soluble peptides for NMR studies", Journal of Biomolecular NMR, Vol 26, No 3, July 2003.
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Source: Addgene
