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URL: http://www.addgene.org/126651
Proper Citation: RRID:Addgene_126651
Insert Name: Lrig1
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:32183906
Vector Backbone Description: Backbone Marker:Sen Wu, Capecchi laboratory; Vector Backbone:pWS-TK6; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Linker between sfGFP and iCre-ERT2 is a mutated P2A sequence where 2 conserved amino acids are changed to Alanines. This mutated sequence does not skip. The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). Thus, the sfGFP fluorescence should be detectable only in cells in which the marker gene expression level is high. Homology arms subcloned from a clone of RPCI-24 C57BL/6J mouse genomic DNA library. Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation. Mice available from the MMRRC (https://www.mmrrc.org/catalog/sds.php?mmrrc_id=69516).
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No rating or validation information has been found for pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TK.
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Source: Addgene
