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URL: http://www.addgene.org/126764
Proper Citation: RRID:Addgene_126764
Insert Name: B-Hypa2SpCas9
Organism: S. pyogenes
Bacterial Resistance: Ampicillin
Defining Citation: PMID:37717069
Vector Backbone Description: Vector Backbone:pX330-like (without U6-sgRNA coding sequence); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: pCbh-3xFLAG-NLS-Blackjack-Hypa2SpCas9-NLS (without U6-sgRNA coding sequence, with silent mutations). Blackjack variants cleave both 20nt and 5'-extended 21nt spacer containing sgRNAs. B-Hypa2SpCas9 shows higher fidelity and target selectivity than its parental increased fidelity variant, Hypa2SpCas9. The lack of sgRNA expression cassette allows for easy testing of various SpCas9 variants with the same sgRNA expressed from a separate plasmid (e.g. pmCherry_gRNA, Addgene# #80457). For detailed information and plasmid usage, please see the associated publication. Please visit https://doi.org/10.1101/2022.08.16.504097 for bioRxiv preprint.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
