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URL: http://www.addgene.org/126774
Proper Citation: RRID:Addgene_126774
Insert Name: eSpCas9-plus
Organism: S. pyogenes
Bacterial Resistance: Kanamycin
Defining Citation: PMID:32144253
Vector Backbone Description: Vector Backbone:pMJ806-like; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: pET-T7-6xHis-MBP-TEV-FLAG-NLS-eSpCas9-plus-NLS eSpCas9-plus cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs. Shows almost the same fidelity and target selectivity as its parental increased fidelity variant, eSpCas9. For detailed information and plasmid usage, please see the associated publication.
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Source: Addgene
