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URL: http://www.addgene.org/127577
Proper Citation: RRID:Addgene_127577
Insert Name: PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:34342575
Vector Backbone Description: Backbone Size:3748; Vector Backbone:pRG203MX; Vector Types:Yeast Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin
Comments: For additional information, please also refer to Azizoğlu et al. (2023) "Autorepression-Based Conditional Gene Expression System in Yeast for Variation-Suppressed Control of Protein Dosage" Curr Protoc. https://doi.org/10.1002/cpz1.647.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org