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URL: http://www.addgene.org/13058
Proper Citation: RRID:Addgene_13058
Insert Name: plcg1
Organism: Danio rerio
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12782653
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3900; Vector Backbone:pCR2.1; Vector Types:; Bacterial Resistance:Ampicillin
Comments: The full-length coding sequence of zebrafish plcg1 was amplified by PCR using cDNA from wild-type embryos using the following oligonucleotides: 5'-TTACTAGTGAACAAACAGGGGAAATG-GCT-3' and 5'-TTTCTAGACTGCTCGGTTTACGCTCGGTTAT-3' and TOPO-cloned into pCR2.1 (Invitrogen) to give pCRplcg1CDS. For riboprobe, digest with MluI and use SP6 polymerase.
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Source: Addgene
