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URL: http://www.addgene.org/131207
Proper Citation: RRID:Addgene_131207
Insert Name: NOD2
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:33942347
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: For cloning Nod2, variant 1 was used. Nod1wt was cut out and Nod2 without stop codon was integrated. To ensure that EGFP and Nod2 are in-frame, two additional bases were added at reverse primer (primer without stop codon of Nod2). Insert has three silent mutations: Codon 178: TCG instead of TCC --> Serine Codon 587: CCT instead of CCC --> Proline Codon 792: CGG instead of CGT --> Arginine
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No rating or validation information has been found for pcDNA5-FRT-TO-NOD2-EGFP.
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Source: Addgene
