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URL: http://www.addgene.org/135188
Proper Citation: RRID:Addgene_135188
Insert Name: CmR
Organism: Synthetic
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:29065183
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Contains two multiple cloning sites for insertion of homologous regions for targeted deletion via lamba red system in E. coli. Contains I-CreI flanking the Cm cassette outside of the flanking multiple cloning sites for excision of homologous regions and CmR marker from the plasmid. Contains I-SceI sites flanking the Cm cassette to allow for excision of the Cm cassette after integration into the E. coli genome for markerless deletion.
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Source: Addgene
