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URL: http://www.addgene.org/136688
Proper Citation: RRID:Addgene_136688
Insert Name: Beclin-1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:29185708
Vector Backbone Description: Vector Backbone:pET21b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Large-scale expression of the BECN1 fusion protein was performed by growing cultures in TB at 37 °C with orbital shaking at 180 rpm to mid-log phase (A600 ∼ 0.6−0.8), cooling cultures to 18 °C for 30 min before induction with 0.5 mM isopropyl β-D-1-thiogalactopyranoside, and growing the induced cultures for 18 h at 18 °C. Cell pellets were harvested via centrifugation and stored at −50 °C. See Ranaghan et al. (2017) [DOI: 10.1021/acs.biochem.7b00936] for purification methods.
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No rating or validation information has been found for BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA].
No alerts have been found for BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA].
Source: Addgene
