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URL: http://www.addgene.org/137044
Proper Citation: RRID:Addgene_137044
Insert Name: Gfap
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:pDTA-5; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin
Comments: A targeting vector to knock-in T2A-td-sfGFP into mouse Gfap protein isoform 2 (also known as Gfap-alpha). This is the most abundant isoform in the mouse brain. Of note, the Gfap protein transcript variant 2 transcript level is high in the SVZ, an adult neural stem cell niche. See Kamphuis et al., 2012 (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0042823). Homology arms subcloned from a clone of RPCI-23 C57BL/6J mouse genomic DNA library. Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.
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No rating or validation information has been found for pB6-Gfap-T2A-td-sfGFP-FNF-DTA.
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Source: Addgene
