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URL: http://www.addgene.org/13786
Proper Citation: RRID:Addgene_13786
Insert Name: mir30 cassette
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:17209010
Vector Backbone Description: Backbone Size:5555; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Cre recombinase-dependent inducible RNAi vector. shRNA is expressed only in the presence of Cre. Haipin DNA can be cloned into the XhoI/EcoRI site. A protocol for hairpin DNA design and cloning was reported by Gregory Hannon lab http://hannonlab.cshl.edu/publications/shrt_hrpin_RNA.pdf (Paddison et al. Nat. Methods 1, 163-167 (2004)). Addgene's quality control sequencing finds a few nucleotides of discrepancy with the depositor's provided full plasmid sequence. These changes are not thought to affect plasmid function.
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Source: Addgene
