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URL: http://www.addgene.org/14020
Proper Citation: RRID:Addgene_14020
Insert Name: Tcf4B
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:14667415
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, avian expression; Bacterial Resistance:Ampicillin
Comments: RCAS dnTcf4en virus was constructed using the mouse Tcf4B splice variant (gift of G. Dressler), as the chick Tcf4B splice variant is expressed in the developing limbs. The dominant-negative (dn) form was created by deleting amino acids 2–50, resulting in the production of a putative dominant-negative protein unable to bind and be activated by beta-catenin, but able to bind DNA via its C-terminal HMG box. The repressor domain from the Drosophila engrailed gene was fused in-frame to the 3′end of the dnTcf4 construct in the shuttle vector pSlax13 and then subcloned into retroviral vector RCAS(BP) A.
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No rating or validation information has been found for RCAS(A) mdnTcf4Ben (CT#1130).
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Source: Addgene
